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kchip2  (Alomone Labs)


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    Structured Review

    Alomone Labs kchip2
    Kchip2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+kchip2/Anti-KChIP2+Antibody/pm41490034-185-39-41
    Average 94 stars, based on 11 article reviews
    kchip2 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Cardiac electrophysiological remodeling associated with enhanced arrhythmia susceptibility in a canine model of elite exercise
    Article Snippet: .. After the incubation period, cells were labeled overnight at 4°C with anti-KChIP2 (Alomone, #APC-142, RRID: AB_2756744 ), anti-Kv4.3 (Alomone, #APC-017, RRID: AB_2040178 ), anti-HCN1 (Alomone, #APC-056, RRID: AB_2039900 ), anti-HCN2 (Alomone, #APC-030, RRID: AB_2313726 ), and anti-HCN4 (Alomone, #APC-052, RRID: AB_2039906 ) primary antibodies diluted 1:50. .. The next day, cells were incubated with goat anti-rabbit IgG Alexa Fluor 488 (ThermoFisher, A-11034, RRID: AB_2576217 ) secondary antibody (dilution: 1:500, ThermoFisher).

    Article Title: Cardiac electrophysiological remodeling associated with enhanced arrhythmia susceptibilty in a canine model of elite exercise
    Article Snippet: .. After the incubation period, cells were labelled overnight at 4°C with anti-KChIP2 (Alomone, #APC-142, RRID:AB_2756744), anti-Kv4.3 (Alomone, #APC-017, RRID:AB_2040178), anti-HCN1 (Alomone, #APC-056, RRID:AB_2039900), anti-HCN2 (Alomone, #APC-030, RRID:AB_2313726) and anti-HCN4 (Alomone, #APC-052, RRID:AB_2039906) primary antibody diluted to 1:50. .. The next day, cells were incubated with goat anti-rabbit IgG Alexa Fluor 488 (ThermoFisher, A-11034, RRID:AB_2576217) secondary antibody (dilution: 1:500, ThermoFisher).

    Labeling:

    Article Title: Cardiac electrophysiological remodeling associated with enhanced arrhythmia susceptibility in a canine model of elite exercise
    Article Snippet: .. After the incubation period, cells were labeled overnight at 4°C with anti-KChIP2 (Alomone, #APC-142, RRID: AB_2756744 ), anti-Kv4.3 (Alomone, #APC-017, RRID: AB_2040178 ), anti-HCN1 (Alomone, #APC-056, RRID: AB_2039900 ), anti-HCN2 (Alomone, #APC-030, RRID: AB_2313726 ), and anti-HCN4 (Alomone, #APC-052, RRID: AB_2039906 ) primary antibodies diluted 1:50. .. The next day, cells were incubated with goat anti-rabbit IgG Alexa Fluor 488 (ThermoFisher, A-11034, RRID: AB_2576217 ) secondary antibody (dilution: 1:500, ThermoFisher).

    Membrane:

    Article Title: Cardiac electrophysiological remodeling associated with enhanced arrhythmia susceptibility in a canine model of elite exercise
    Article Snippet: .. The membrane was blocked with 2.5% non-fat milk for 1 hr at room temperature and immunolabeled overnight at 4°C with anti-KChIP2 (Alomone, #APC-142, RRID: AB_2756744 ) and anti-Kv4.3 (Alomone, #APC-017, RRID: AB_2040178 ) primary antibodies diluted 1:1000. .. This was followed by incubation for 1 hr with Goat anti-Rabbit IgG-HRP (SouthernBiotech, 4030–05, RRID: AB_2687483 ) secondary antibody at a dilution of 1:8000.

    Article Title: Cardiac electrophysiological remodeling associated with enhanced arrhythmia susceptibilty in a canine model of elite exercise
    Article Snippet: .. The membrane was blocked with 2.5% non-fat milk for 1 hour at room temperature and immunolabelled overnight at 4°C with anti-KChIP2 (Alomone, #APC-142, RRID:AB_2756744) and anti-Kv4.3 (Alomone, #APC-017, RRID:AB_2040178) primary antibody diluted to 1:1000. .. This was followed by 1 hour incubation with Goat anti-Rabbit IgG-HRP (SouthernBiotech, 4030-05, RRID:AB_2687483) secondary antibody in a dilution of 1:8000.

    Immunolabeling:

    Article Title: Cardiac electrophysiological remodeling associated with enhanced arrhythmia susceptibility in a canine model of elite exercise
    Article Snippet: .. The membrane was blocked with 2.5% non-fat milk for 1 hr at room temperature and immunolabeled overnight at 4°C with anti-KChIP2 (Alomone, #APC-142, RRID: AB_2756744 ) and anti-Kv4.3 (Alomone, #APC-017, RRID: AB_2040178 ) primary antibodies diluted 1:1000. .. This was followed by incubation for 1 hr with Goat anti-Rabbit IgG-HRP (SouthernBiotech, 4030–05, RRID: AB_2687483 ) secondary antibody at a dilution of 1:8000.



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    Effects of NS5806 on I to channel-related gene and protein expression. ( A – C ) Quantification of the mRNA levels of Kv4.2 ( A ), <t>KChIP2</t> ( B ) and DPP6 ( C ). GAPDH served as an internal control (n = 9 in each group). ( D ) Representative immunoblots. Original blots are presented in Supplementary Figure . ( E – G ) Summary data of Kv4.2 ( E ), KChIP2 ( F ) and DPP6 ( G ) protein levels. GAPDH served as an internal control (n = 5 in each group). * P < 0.05, ** P < 0.01 versus the Sham + Vehicle group. # P < 0.05, ## P < 0.01 versus TAC + Vehicle.
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    Protein profiles of key potassium channels and sex hormone receptors in hearts of aged Akita mice. Key potassium channels and their auxiliary proteins such as <t>KChIP2,</t> Kv1.4, Kv1.5, and Kv4.2 and housekeeping gene GAPDH ( a ), and androgen receptor (AR) and estrogen receptors (ER-α and β) ( b ). Values shown in the tables are mean (±SE) normalized band intensities from n = 4 per group. * represents p < 0.05 and ** p < 0.005 between normoxia and hyperoxia.
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    Image Search Results


    Effects of NS5806 on I to channel-related gene and protein expression. ( A – C ) Quantification of the mRNA levels of Kv4.2 ( A ), KChIP2 ( B ) and DPP6 ( C ). GAPDH served as an internal control (n = 9 in each group). ( D ) Representative immunoblots. Original blots are presented in Supplementary Figure . ( E – G ) Summary data of Kv4.2 ( E ), KChIP2 ( F ) and DPP6 ( G ) protein levels. GAPDH served as an internal control (n = 5 in each group). * P < 0.05, ** P < 0.01 versus the Sham + Vehicle group. # P < 0.05, ## P < 0.01 versus TAC + Vehicle.

    Journal: Scientific Reports

    Article Title: The Kv4 potassium channel modulator NS5806 attenuates cardiac hypertrophy in vivo and in vitro

    doi: 10.1038/s41598-024-70962-x

    Figure Lengend Snippet: Effects of NS5806 on I to channel-related gene and protein expression. ( A – C ) Quantification of the mRNA levels of Kv4.2 ( A ), KChIP2 ( B ) and DPP6 ( C ). GAPDH served as an internal control (n = 9 in each group). ( D ) Representative immunoblots. Original blots are presented in Supplementary Figure . ( E – G ) Summary data of Kv4.2 ( E ), KChIP2 ( F ) and DPP6 ( G ) protein levels. GAPDH served as an internal control (n = 5 in each group). * P < 0.05, ** P < 0.01 versus the Sham + Vehicle group. # P < 0.05, ## P < 0.01 versus TAC + Vehicle.

    Article Snippet: Primary antibodies against Kv4.2 (1:500, 75–016, NeuroMab, USA), KChIP2 (1:500, DF14620, Affinity, China), DPP6 (1:200, APC-146, Alomone, Israel) and GAPDH (1:10,000, 10494-1-AP, Proteintech, China) were incubated at 4 °C overnight.

    Techniques: Expressing, Control, Western Blot

    Protein profiles of key potassium channels and sex hormone receptors in hearts of aged Akita mice. Key potassium channels and their auxiliary proteins such as KChIP2, Kv1.4, Kv1.5, and Kv4.2 and housekeeping gene GAPDH ( a ), and androgen receptor (AR) and estrogen receptors (ER-α and β) ( b ). Values shown in the tables are mean (±SE) normalized band intensities from n = 4 per group. * represents p < 0.05 and ** p < 0.005 between normoxia and hyperoxia.

    Journal: Cells

    Article Title: Influence of Age on Hyperoxia-Induced Cardiac Pathophysiology in Type 1 Diabetes Mellitus (T1DM) Mouse Model

    doi: 10.3390/cells12111457

    Figure Lengend Snippet: Protein profiles of key potassium channels and sex hormone receptors in hearts of aged Akita mice. Key potassium channels and their auxiliary proteins such as KChIP2, Kv1.4, Kv1.5, and Kv4.2 and housekeeping gene GAPDH ( a ), and androgen receptor (AR) and estrogen receptors (ER-α and β) ( b ). Values shown in the tables are mean (±SE) normalized band intensities from n = 4 per group. * represents p < 0.05 and ** p < 0.005 between normoxia and hyperoxia.

    Article Snippet: Blots were later blocked in 5% w / v nonfat dry milk for 1 h and probed in 1:5000 dilutions of primary antibodies for Kv1.4 (AB5926) and GAPDH (Millipore) antibodies, and 1:1000 dilutions of KChIP2 (Abcam, Cambridge, MA, USA), Kv4.2 (Millipore, Billerica, MA, USA), estrogen receptor beta (Thermo Fisher, CA, USA), androgen receptor (Abcam, Cambridge, MA, USA), and Kv1.5 antibody (Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: